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Nanoscale Protein Analysis

ProteinSimple Capillary Immunoassay Technologies (Building 37)

The Collaborative Protein Technologies Resource (CPTR) has extensive experience in the characterization of proteins for post-translational modifications and can assist PI's with the Nanopro isoelectric focusing immunoassays which can profile in 6-24 ng of total cell lysate protein the percent phosphorylation status of a given protein using antibody specific to the unmodified protein.  This extremely sensitive technique requires validated antibodies and the CPTR will provide the validation services as well as the actual performance of the assays.  Some consumables will be required from the researcher.

Simple Western Assays, Capillary based, fully automated Western system, highly quantitative data with excellent reproducibility

Log on to the CPTR Website and Project Request System

If you are using an Apple computer, you will need to use the Safari Browser to access the LabShare System.  Firefox will not work.

Contact information:

Jinqui (Jessie) Chen:
chenj13@mail.nih.gov
Telephone: 301-496-7917

Please click on the link below to download a summary of the CPTR capabilities.

PI retreat handout.ppt

NanoPro/Peggy Sue capillaries are supported with OSTR subsidy (50%)

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