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High-Content Imaging

HighThroughput Imaging Facility (HiTIF)

The NCI High-Throughput Imaging Facility (HiTIF) provides infrastructure and expertise to NCI intramural scientists for automated microscopy assay development, discovery of small molecule compounds, and characterization of cellular pathways using RNAi-based screens. In addition to screening capabilities, the facility develops high-throughput microscopy analytical methods that provide unique insights into the mechanisms underlying rare cellular events within adherent monolayer cell populations. The facility may also collaborate with end-users to develop and execute smaller scale projects that utilize automated fluorescence microscopy technologies.

Potential projects from NCI/CCR investigators are approved by the HiTIF Oversight Committee, which is chaired by Dr. Carole Parent, Laboratory of Cellular and Molecular Biology. To initiate a project with the HiTIF, the investigator first submits a short proposal to the facility head. This document should explain the goals of the project, potential scientific significance, and preliminary data as proof-of-concept.  

Established Technologies:
  • Fully automated, high-throughput fluorescence microscopy (Perkin Elmer Opera instruments)
  • Automated image analysis to measure specific cellular features, molecular localization, etc
  • Assistance with miniaturization of cell-based assays in 96-well and 384-well microplates
  • Robotic sample preparation, reagent addition, and plate washing
  • Discovery of small molecule compounds via cell biological read-outs
  • Characterization of cellular mechanistic pathways using RNAi-based screens

Contact Information:

Gianluca Pegoraro, Ph.D.
Facility Head, High-Thoughput Imaging Facility
41 Library Drive, Building 41, Room B622
Bethesda, MD 20892
Phone: 240-760-6696

OSTR provides 50% subsidy towards smaller, pilot projects through the HiTIF.  Subsidies can be requested through the OSTR Subsidy website.  Subsidies for larger projects will be considered through the RRS (Resource Request System). When logged into the RRS select "OSTR Technology Supplement."  


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