Skip to main content

Next Generation Sequencing Platforms

CCR investigators have access to a variety of DNA sequencing services in both Frederick and Bethesda.

Genomics Laboratory at the Cancer Research Technology Program (Frederick, MD)

Sequencing and Automation
 
  • Variety of options for NGS library preparations, including substantial project design consultation, Ion Torrent (CLIA certified), and Illumina MiSeq
  • Whole exome capture using Agilent’s SureSelect reagents
  • 16s Microbiome Pipeline: Automated fecal sample extraction, library preparation, normalization, and sequencing on MiSeq for bacterial 16s RNA gene.
  • Specific single nucleotide polymorphism (SNP) detection and DNA methylation analysis on the Qiagen Pyromark platform
  • Large projects requiring laboratory automation are managed using one of several Beckman BioMek FX liquid handling systems.

For more information contact:
Xiaolin Wu, Ph.D.
Genomics Technology Laboratory
Phone: 301-846-7677
E-mail: forestwu@mail.nih.gov

CCR Genomics Core in Building 37, Bethesda

Sanger Sequencing :

  • Rapid, accurate and affordable sequencing of DNA samples and high-throughput projects utilizing ABI 3130xl and 3730xl platforms. 

Next-Generation Sequencing:

• Viral and bacterial genomes sequencing

• T-Cell and B-Cell receptor sequencing

• RNAseq

• Ampliconsequencing

• Custom projects

Additional NGS equipment:

            • Agilent 2200 Tapestation–QC platform

            • Sage Science PippinHT-high throughput sizing 

 

For more information contact:

Elizabeth A. Conner, Ph.D.                                                  Val Bliskovsky, Ph.D.

Facility Head, CCR Genomics Core                                      Genomics Expert, CCR Genomics Core

Bldg. 37, Rm 2135                                                              Bldg. 37, Rm 2135

Bethesda, MD 20892                                                           Bethesda, MD 20892

Phone: 240-760-7373                                                        Phone: 240-760-7363

E-mail: liz_conner@nih.gov                                                 E-mail: BliskovV@mail.nih.gov

 

CCR Sequencing Facility

Click Here to get to submit a sequencing request

Provides state-of-the-art, high-throughput sequencing technologies to identify single nucleotide polymorphisms, insertions and deletions, and copy number or structural variations.

The SF offers sequencing on two platforms:

Illumina NextSeq500, HiSeq2500, and HiSeq2000 sequencing platforms

  • Sequencing utilizes reversible terminator chemistry optimized to achieve high levels of cost effectiveness and throughput (Detailed description)
  • Millions of reads produced per sample lane at 36bp and 101bp read lengths
  • Support for the multiplexing of bar-coded samples into a single lane
  • Available resources include two HiSeq 2000s, two NextSeq 500s, and one HiSeq2500 sequencers

PacBio RS sequencing

  • Sequencing via single molecule real-time (SMRT) technology allows rapid identification of long nucleotide chains (Detailed description)
  • Read lengths averaging 1,800bp per molecule facilitate genome assembly and mapping of repetitive regions
  • Minimizes machine turnaround time and provides flexibility in experimental and run design
  • As a selected member of the Limited Pre-Release program, installation of the PacBio RS prior to commercial release has made the Sequencing Facility a unique source of this new technology

Contact information:

Bao Tran
Laboratory Director
ATRF Room D-3047
Phone: 301-360-3460
Fax: 301-360-3465
tranb2@mail.nih.gov

Please inquire with the OSTR for information about subsidies for sequencing services.

 

Go to top